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抗ERp72抗体

  • 新陈代谢
  • 发布时间:2023-10-25 14:46   咨询量:
  • 英文名称: anti- ERp72 antibody
  • 产品货号: FNab02859
  • 适应种属:人源,大鼠,小鼠
  • 宿主:兔
  • 亚型:lgG
  • 验证应用: ELISA, WB, IHC, IF, IP
  • 简介:菲恩生物抗ERp72抗体经SDS-PAGE分析,纯度大于95%,采用免疫亲和纯化方法,为用户提供高亲和力、高特异性的优质抗体。
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    产品详情
    名称: anti- ERp72 antibody
    货号: FNab02859
    适应种属:人源,大鼠,小鼠
    规格:100ug(微克)
    状态:液体
    纯化方法:免疫亲和纯化
    纯度:经SDS-PAGE分析,纯度大于95%
    宿主:
    亚型:lgG
    存储:用添加了0.02%叠氮化钠和50%甘油的PBS溶解,PH值7.3,可在-20℃下保存12个月(避免反复冻融)
    免疫原: protein disulfide isomerase family A, member 4
    免疫原别称: ER protein 70 antibody, ER protein 72 antibody, ERp 72 antibody, ERP70 antibody, ERP72 antibody, PDIA4 antibody, Protein disulfide isomerase A4 antibody
    分子量: 72 kDa
    验证应用: ELISA, WB, IHC, IF, IP
    建议稀释比例: WB: 1:500-1:2000; IP: 1:500-1:1000; IHC: 1:20-1:200; IF: 1:20-1:200
    UniProt ID: P13667
    克隆性:多克隆
    背景介绍:PDIA4(Protein disulfide-isomerase A4) is also named as ERP70, ERP72 and belongs to the protein disulfide isomerase family. It catalyzes the rearrangement of-S-S-bonds in proteins. ERp72 is a soluble protein localized in the ER lumen and contains the COOH-terminal retention signal, KEEL. There are 6 cysteine residues in the amino acid sequences of mouse and human ERp72. All of the cysteine residues occur in the internal thioredoxin motif, CGHC(PMID:15475357).
    图像:ERp72抗体
    Immunofluorescent analysis of Hela cells, using ERp72 antibody FNab02859 at 1:50 dilution and FITC-labeled goat anti-rabbit IgG (green). Blue pseudocolor = DAPI (fluorescent DNA dye)。

         ERp72抗体
    Immunohistochemistry of paraffin-embedded human lung cancer using FNab02859(PDIA4 antibody) at dilution of 1:100。


         anti- ERp72 antibody
    IP Result of anti-ERp72 (IP: FNab02859, 4ug; Detection: FNab02859 1:500) with HeLa cells lysate 2800ug。


    anti- ERp72 antibody
    Various lysates were subjected to SDS PAGE followed by western blot with FNab02859(PDIA4 antibody) at dilution of 1:500。

    

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